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 | Acesso ao texto completo restrito à biblioteca da Embrapa Trigo. Para informações adicionais entre em contato com cnpt.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Trigo. |
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Data corrente: |
21/02/2022 |
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Data da última atualização: |
05/09/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROESLER, E. A.; BALBINOTT, N.; SCHROEDER, R. G.; LAU, E. Y.; LIMA, M. I. P. M.; BRAMMER, S. P.; ARAGÃO, F. J. L.; FERNANDES, J. M. C.; DEUNER, C. C. |
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Afiliação: |
EDUARDO ANDRÉ ROESLER, UPF; NATALIA BALBINOTT, UFRGS; RENATA GABRIELA SCHROEDER, UPF; ELENE YAMAZAKI LAU, CNPT; MARIA IMACULADA PONTES M LIMA, CNPT; SANDRA PATUSSI BRAMMER, CNPT; FRANCISCO JOSE LIMA ARAGAO, Cenargen; JOSE MAURICIO CUNHA FERNANDES, CNPT; CAROLINA CARDOSO DEUNER, UPF. |
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Título: |
An efficient protocol for inoculation of Fusarium graminearum in detached leaves of Arabidopsis. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Tropical Plant Pathology, 16 Feb. 2022 |
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DOI: |
https://doi.org/10.1007/s40858-022-00497-x |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Fusarium graminearum is the main cause of Fusarium head blight, a fungal disease that reduces yield and afects the quality of wheat and other small grains. Genetic resistance is the ideal method for disease control; however, there are not yet cultivars with sufcient resistance levels to withstand an epidemic. Genetic engineering strategies such as gene silencing, host-induced gene silencing (HIGS), overexpression, and genome editing are promising, but many target genes need to be analyzed to fnd a suitable one. Arabidopsis thaliana is a model plant that is also susceptible to F. graminearum. Although this interaction was reported nearly two decades ago, consistent infection and symptoms are not always obtained. The availability of an efcient inoculation method is essential for studying plant-pathogen interaction. This work aimed at testing protocols for inoculating F. graminearum in detached leaves of A. thaliana which varied in inoculation site (leaf sides), wound (abrasion or hole injury), and inoculum type (mycelium agar disk or spore). We found that a mycelium agar disk placed on an abrasion injury at the adaxial leaf side was the most efcient (highest lesion size, incidence, and easiness to visualize the symptoms caused by the pathogen) method to inoculate F. graminearum in detached leaves of Arabidopsis. |
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Palavras-Chave: |
Inoculation assay; Protocol. |
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Thesagro: |
Doença Fúngica; Ferrugem; Fusarium Graminearum; Trigo. |
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Thesaurus Nal: |
Arabidopsis thaliana; Fusarium head blight. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02292naa a2200325 a 4500
001 2140281
005 2022-09-05
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s40858-022-00497-x$2DOI
100 1 $aROESLER, E. A.
245 $aAn efficient protocol for inoculation of Fusarium graminearum in detached leaves of Arabidopsis.$h[electronic resource]
260 $c2022
520 $aAbstract: Fusarium graminearum is the main cause of Fusarium head blight, a fungal disease that reduces yield and afects the quality of wheat and other small grains. Genetic resistance is the ideal method for disease control; however, there are not yet cultivars with sufcient resistance levels to withstand an epidemic. Genetic engineering strategies such as gene silencing, host-induced gene silencing (HIGS), overexpression, and genome editing are promising, but many target genes need to be analyzed to fnd a suitable one. Arabidopsis thaliana is a model plant that is also susceptible to F. graminearum. Although this interaction was reported nearly two decades ago, consistent infection and symptoms are not always obtained. The availability of an efcient inoculation method is essential for studying plant-pathogen interaction. This work aimed at testing protocols for inoculating F. graminearum in detached leaves of A. thaliana which varied in inoculation site (leaf sides), wound (abrasion or hole injury), and inoculum type (mycelium agar disk or spore). We found that a mycelium agar disk placed on an abrasion injury at the adaxial leaf side was the most efcient (highest lesion size, incidence, and easiness to visualize the symptoms caused by the pathogen) method to inoculate F. graminearum in detached leaves of Arabidopsis.
650 $aArabidopsis thaliana
650 $aFusarium head blight
650 $aDoença Fúngica
650 $aFerrugem
650 $aFusarium Graminearum
650 $aTrigo
653 $aInoculation assay
653 $aProtocol
700 1 $aBALBINOTT, N.
700 1 $aSCHROEDER, R. G.
700 1 $aLAU, E. Y.
700 1 $aLIMA, M. I. P. M.
700 1 $aBRAMMER, S. P.
700 1 $aARAGÃO, F. J. L.
700 1 $aFERNANDES, J. M. C.
700 1 $aDEUNER, C. C.
773 $tTropical Plant Pathology, 16 Feb. 2022
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Registro original: |
Embrapa Trigo (CNPT) |
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Registro Completo
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Biblioteca(s): |
Embrapa Clima Temperado. |
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Data corrente: |
07/12/2020 |
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Data da última atualização: |
07/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
PEREIRA, A. M.; BONEMANN, D. H.; SCHERDIEN, S. H.; ÁVILA, B. P.; ANTUNES, I. F.; RIBEIRO, A. S.; GULARTE, M. A. |
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Afiliação: |
ALINE MACHADO PEREIRA, UFPEL; DAISA HAKBART BONEMANN, UFPEL; SABRINA HÄRTER SCHERDIEN, UFPEL; BIANCA PIO ÁVILA, UFPEL; IRAJA FERREIRA ANTUNES, CPACT; ANDERSON SCHWINGEL RIBEIRO, UFPEL; MARCIA AROCHA GULARTE, UFPEL. |
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Título: |
Evaluation of total and bioaccessible concentration of minerals in creole beans. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Journal of Food Composition and Analysis, v. 94, 103622, Dec. 2020. |
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ISSN: |
0889-1575 |
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DOI: |
doi.org/10.1016/j.jfca.2020.103622 |
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Idioma: |
Inglês |
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Conteúdo: |
The present study evaluated the total and bioaccessible concentrations of minerals in raw and cooked Creole beans. An analytical methodology was optimized, which involved several steps from sample preparation, accuracy study, data treatment, and instrumental analysis ? using a microwave-induced plasma optical emission spectrometer (MIP OES). The present study provides information about the total concentration of the metals Ba, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Pb, Zn and the bioaccessible fraction of metals Ba, Cu, Fe, Mn, Pb and Zn in three different types of creole beans. The elements that presented a greater quantity on all beans, both raw and cooked, were K and Mg. K varied from 12,600 to 13,300 mg/kg for the raw beans and 4590?10300 mg/kg for the cooked beans. Mg varied between 1620 and 1980 mg/kg for the raw beans and 670?1040 mg/kg for the cooked beans. The BRS Expedito type showed a higher concentration of K and Mg on raw and cooked beans. However, when the bioaccessibility study was carried out among the elements listed above, the most bioaccessible were Cu and Zn. Cu presented a bioaccessible fraction percentage between 52.6?59.2% for raw beans and 38.5?61.2% for cooked beans. Zn presented a bioaccessible fraction percentage between 45.6?50.6% for raw beans and 43%?63.5% for cooked beans. The results found on this study indicate that, possibly, the water and the cooking process significantly influences the mineral content. Also, studying mineral concentration on cooked beans is more nutritionally important than characterizing the raw grain, as beans are a type of food mostly consumed on its cooked form. MenosThe present study evaluated the total and bioaccessible concentrations of minerals in raw and cooked Creole beans. An analytical methodology was optimized, which involved several steps from sample preparation, accuracy study, data treatment, and instrumental analysis ? using a microwave-induced plasma optical emission spectrometer (MIP OES). The present study provides information about the total concentration of the metals Ba, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Pb, Zn and the bioaccessible fraction of metals Ba, Cu, Fe, Mn, Pb and Zn in three different types of creole beans. The elements that presented a greater quantity on all beans, both raw and cooked, were K and Mg. K varied from 12,600 to 13,300 mg/kg for the raw beans and 4590?10300 mg/kg for the cooked beans. Mg varied between 1620 and 1980 mg/kg for the raw beans and 670?1040 mg/kg for the cooked beans. The BRS Expedito type showed a higher concentration of K and Mg on raw and cooked beans. However, when the bioaccessibility study was carried out among the elements listed above, the most bioaccessible were Cu and Zn. Cu presented a bioaccessible fraction percentage between 52.6?59.2% for raw beans and 38.5?61.2% for cooked beans. Zn presented a bioaccessible fraction percentage between 45.6?50.6% for raw beans and 43%?63.5% for cooked beans. The results found on this study indicate that, possibly, the water and the cooking process significantly influences the mineral content. Also, studying mineral concentration ... Mostrar Tudo |
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Palavras-Chave: |
Feijão crioulo. |
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Thesagro: |
Análise de Alimento; Feijão; Grão; Nutriente Mineral. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/218892/1/Artigo-Evaluation-of-total-and-bioaccessible-concentration.pdf
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Marc: |
LEADER 02459naa a2200277 a 4500
001 2127769
005 2020-12-07
008 2020 bl uuuu u00u1 u #d
022 $a0889-1575
024 7 $adoi.org/10.1016/j.jfca.2020.103622$2DOI
100 1 $aPEREIRA, A. M.
245 $aEvaluation of total and bioaccessible concentration of minerals in creole beans.$h[electronic resource]
260 $c2020
520 $aThe present study evaluated the total and bioaccessible concentrations of minerals in raw and cooked Creole beans. An analytical methodology was optimized, which involved several steps from sample preparation, accuracy study, data treatment, and instrumental analysis ? using a microwave-induced plasma optical emission spectrometer (MIP OES). The present study provides information about the total concentration of the metals Ba, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Pb, Zn and the bioaccessible fraction of metals Ba, Cu, Fe, Mn, Pb and Zn in three different types of creole beans. The elements that presented a greater quantity on all beans, both raw and cooked, were K and Mg. K varied from 12,600 to 13,300 mg/kg for the raw beans and 4590?10300 mg/kg for the cooked beans. Mg varied between 1620 and 1980 mg/kg for the raw beans and 670?1040 mg/kg for the cooked beans. The BRS Expedito type showed a higher concentration of K and Mg on raw and cooked beans. However, when the bioaccessibility study was carried out among the elements listed above, the most bioaccessible were Cu and Zn. Cu presented a bioaccessible fraction percentage between 52.6?59.2% for raw beans and 38.5?61.2% for cooked beans. Zn presented a bioaccessible fraction percentage between 45.6?50.6% for raw beans and 43%?63.5% for cooked beans. The results found on this study indicate that, possibly, the water and the cooking process significantly influences the mineral content. Also, studying mineral concentration on cooked beans is more nutritionally important than characterizing the raw grain, as beans are a type of food mostly consumed on its cooked form.
650 $aAnálise de Alimento
650 $aFeijão
650 $aGrão
650 $aNutriente Mineral
653 $aFeijão crioulo
700 1 $aBONEMANN, D. H.
700 1 $aSCHERDIEN, S. H.
700 1 $aÁVILA, B. P.
700 1 $aANTUNES, I. F.
700 1 $aRIBEIRO, A. S.
700 1 $aGULARTE, M. A.
773 $tJournal of Food Composition and Analysis$gv. 94, 103622, Dec. 2020.
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